Serum biochemical profile and molecular detection of pathogens in semen of infertile male dromedary camels (Camelus dromedarius)

Published Date
Journal
American Reproduction Science
Citation
Animal Reproduction Science Volume 180, May 2017, Pages 58-65
DOI
10.1016/j.anireprosci.2017.03.003
Authors
Al-Busadaha AK
El-Bahrb SM
Khalafallad AI
Abstract

Detection of pathogens in the semen of camels has not been completely elucidated. There-fore, the current study aimed to determine the association of some economically important pathogens with infertility in 94 male infertile camels through molecular detection and estimation of selected biochemical parameters in serum of these animals compared with a control non infected fertile animals (n = 40). PCR analysis of semen samples of infer-tile camels indicated that, four potential pathogens namely Mycoplasma spp., Leptospiraspp., Brucella melitensis, and Bovine viral diarrhea virus (BVDV) were detected in 50 semen samples of infertile camels whereas, 44 semen samples of infertile camels were free of pathogens and all tested semen samples were negative for bovine herpes virus 1, Salmonellaspp. and Trypanosoma evansi. Single and mixed infection was detected in 88% and 12% of the infected semen samples, respectively. Mycoplasma spp., Leptospira spp., Brucella andBovine viral diarrhea virus infection represented 66%, 27.2%, 4.5% and 2.3% of the single infected semen samples. Mycoplasma spp. + Leptospira spp. and Mycoplasma spp. + Brucellaspp. were detected in 83.3% and 16.7% of mixed infected semen samples, respectively.Testosterone concentration decreased significantly in infertile infected camels compare to both control and infertile non infected animals that remained comparable. The current findings reported the molecular detection of mixed infection in camel semen for the first time. Mycoplasma spp. is the most widely recognized microorganism in the present study and together with Leptospira spp., Brucella spp. and Bovine viral diarrhea virus, might be associated with infertility in dromedary camels.