An Adsorption Procedure to Increase the Specificity of Enzyme-Linked Immunosorbent Assays for Lyme disease without Decreasing Sensitivity

Published Date
Journal
Arthritis and Rheumatism
Citation
Arthritis Rheum. 1989 Aug;32(8):1041-4.
DOI
10.1002/anr.1780320814
Authors
Fawcett PT
Doughty RA
O'Brien AE
Abstract

Previous efforts to use adsorption techniques to enhance the specificity of enzyme-linked immunosorbent assays for antibodies to the spirochete that causes Lyme disease resulted in unacceptable reductions in assay sensitivity. We present here evidence that preadsorption of serum with Escherichia coli can enhance test specificity without significantly reducing test sensitivity.