Multi-platform Approach for Microbial Biomarker Identification Using Borrelia burgdorferi as a Model

Published Date
Journal
Frontiers in Cellular and Infection Microbiology
Citation
Front Cell Infect Microbiol. 2019 Jun 11;9:179
DOI
10.3389/fcimb.2019.00179
Authors
Pflughoeft KJ
Mash M
Hasenkampf NR
Jacobs MB
Tardo AC
Magee DM
Song L
LaBaer J
Philipp MT
Embers ME
AuCoin DP
Abstract

The identification of microbial biomarkers is critical for the diagnosis of a disease early during infection. However, the identification of reliable biomarkers is often hampered by a low concentration of microbes or biomarkers within host fluids or tissues. We have outlined a multi-platform strategy to assess microbial biomarkers that can be consistently detected in host samples, using Borrelia burgdorferi, the causative agent of Lyme disease, as an example. Key aspects of the strategy include the selection of a macaque model of human disease, in vivo Microbial Antigen Discovery (InMAD), and proteomic methods that include microbial biomarker enrichment within samples to identify secreted proteins circulating during infection. Using the described strategy, we have identified 6 biomarkers from multiple samples. In addition, the temporal antibody response to select bacterial antigens was mapped. By integrating biomarkers identified from early infection with temporal patterns of expression, the described platform allows for the data driven selection of diagnostic targets.