Improved culture conditions for the growth and detection of Borrelia from human serum

Published Date
Journal
International Journal of Medical Sciences
Citation
Int J Med Sci. 2013;10(4):362-7
DOI
10.7150/ijms.5698
Authors
Sapi E
Pabbati N
Datar A
Davies EM
Rattelle A
Kuo BA
Abstract

In this report we present a method to cultivate Borrelia spirochetes from human serum samples with high efficiency. This method incorporates improved sample collection, optimization of culture media and use of matrix protein. The method was first optimized utilizing Borrelia laboratory strains, and later by demonstrating growth of Borrelia from sera from fifty seropositive Lyme disease patients followed by another cohort of 72 Lyme disease patients, all of whom satisfied the strict CDC surveillance case definition for Lyme disease. The procedure resulted in positive cultures in 47% at 6 days and 94% at week 16. Negative controls included 48 cases. The positive identification of Borrelia was performed by immunostaining, PCR, and direct DNA sequencing.